Bacteriological Analysis Of Well Water In New Haven, In Enugu Urban Area

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ABSTRACT

Samples of well water were collected from four (4) different wells  located in different parts of New Haven in Enugu urban area, for  bacteriological analysis. Total bacterial counts, using pour plate  method were carried out. Nutrient agar plates were used for  general bacterial count, while MacConkey agar plates were used  for total coliform count. The four different wells that were  examined showed different bacterial counts. A total of four  different bacteria were isolated.  The bacteria isolated and  identified included: Escherichia coli, Salmonella spp, Staphylococcus aureus, and Bacillus spp.

TABLE OF CONTENTS
Title page
Certification
Dedication
Acknowledgement     
Table of content                                                                    
Abstract      

CHAPTER ONE
Introduction

CHAPTER TWO
Literature review
Contamination of water
Coliform Bacteria
Sources of coliform bacteria
Indicators of fecal contamination
Water borne diseases
Pathogens isolated in sewage and other water
Prevention of well against contamination
Standard Drinking water
Methods of evaluating the bacteriological 
characteristics of water
The most probable number and biochemical Oxygen 
Demand

CHAPTER THREE
Materials and methods
Study area and sampling
Sample collection.
Media and media preparation
Macroscopic examination of water samples
Determination of bacterial contamination and total coliform count using pour plate method.
Identification of isolates
Gram staining
Motility test
Biochemical Test
Washing and sterilization of glass wares.

CHAPTER FOUR
Results

CHAPTER FIVE
Discussion
Conclusion and Recommendation
References
Appendix














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