Effects Of Crude And Citrullus Lanatus Seed Oil On Some Heamatological Indices (pcv, Wbc, Rbc And Platelets) Of Wistar Albino Rats

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ABSTRACT

This study investigated the possible effects of n- hexane extract of crude and degummed Citrullus lanatus seed oil on RBC, WBC, PCV, and Platelet level on wistar albino rats. The result, showed no significant increase in PCV, RBC, WBC and Platelet levels in rats fed with crude and degummed Citrullus lanatus seed oil as well as the control.
The study showed that rats fed with the crude Citrullus lanatus seed oil had a non significant increase when compared with the degummed Citrullus lanatus seed oil and the control in all the parameters checked for. The non significant values could be as a result of the concentrations given. So if the concentration is increased there might be a significant increase in the parameters
In conclusion, n- Hexane extract of crude and degummed Citrullus lanatus seed oil has little or no effect on the PCV but has effect on the RBC. The crude seed oil causes an increase in WBC count probably by increasing monocytes and eosinophils in the blood as well as in Platelet.


TABLE OF CONTENT
CHAPTER ONE
1.0 INTRODUCTION 
1.1 HAEMATOLOGICAL PARAMETERS
1.1.1 Red Blood Cells
1.1.2 White Blood Cells 
1.1.3 Platelets
1.1.4 Haemoglobin.
1.1.5 Packed Cell Volume.
1.2  HAEMATOLOGICAL DISORDERS
1.2.1   Sickle Cell Anaemia
1.2.1.1 Common Morbid Complications
1.2.2      Leukaemia
1.3 STATEMENT OF PROBLEM
1.4 AIM OF THE STUDY
1.5 OBJECTIVES OF THE STUDY
1.6 JUSTIFICATION FOR THE EXPERIMENT
1.7 SCOPE OF THE EXPERIMENT

CHAPTER TWO
LITERATURE REVIEW
2.0 INTRODUCTION
2.1 ORIGIN AND BOTANICAL DESCRIPTION
2.2 NUTRIENT COMPOSTION OF CITRULLUS LANATUS
2.2.1 Nutritional Values
2.2.2 Ethno medicinal Relevance 
2.3 BIOACTIVITIES OF CITRULLUS LANATUS 
2.3.1Antioxidant, Anti-inflammatory and Analgesic Potential 
2.3.2 Laxative Activity
2.3.3 Antimicrobial Activity
2.4.4 Anti-Hyperlipidemic
2.3.5 Antisecretory Effects

CHAPTER THREE
MATERIALS AND METHODS
3.1 MATERIALS AND METHODS
3.1.1 Materials
3.1.2 Equipments Used
3.1.3 Reagents Used 
3.1.4 Plant Material 
3.2 Extraction of Oil
3.3 Degumming of Oil
3.4 Animal Model
3.5 Experimental Design
3.6 PLASMA ANALYSIS
3.6.1 Collection of Blood Sample
3.7 ANALYSIS OF BLOOD SAMPLE
3.7.1 Determination of Total White Blood Cells (TWBC) Count by Haemocytometry
3.7.2 Packed Cell Volume (PCV) estimation
3.7.3 Determination of Red Blood Cells Count by Haemocytometry
3.7.4 Platelet Determination
3.8 Statistical Analysis 
 
CHAPTER FOUR
RESULTS AND ANALYSIS
4.1 Level of Packed Cell Volume
4.2 Level of White Blood Cell
4.3 Level of Red Blood Cell
4.4 Level of Platelet
4.5 DISCUSSION 

CHAPTER FIVE
5.0 RECOMMENDATION
5.1 CONCLUSION
 
REFERENCES

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(2016, 04). Effects Of Crude And Citrullus Lanatus Seed Oil On Some Heamatological Indices (pcv, Wbc, Rbc And Platelets) Of Wistar Albino Rats.. ProjectStoc.com. Retrieved 04, 2016, from https://projectstoc.com/read/7533/effects-of-crude-and-citrullus-lanatus-seed-oil-on-some-heamatological-indices-pcv-wbc-rbc-and-platelets-of-wistar-albino-rats-2668
"Effects Of Crude And Citrullus Lanatus Seed Oil On Some Heamatological Indices (pcv, Wbc, Rbc And Platelets) Of Wistar Albino Rats." ProjectStoc.com. 04 2016. 2016. 04 2016 <https://projectstoc.com/read/7533/effects-of-crude-and-citrullus-lanatus-seed-oil-on-some-heamatological-indices-pcv-wbc-rbc-and-platelets-of-wistar-albino-rats-2668>.
"Effects Of Crude And Citrullus Lanatus Seed Oil On Some Heamatological Indices (pcv, Wbc, Rbc And Platelets) Of Wistar Albino Rats.." ProjectStoc.com. ProjectStoc.com, 04 2016. Web. 04 2016. <https://projectstoc.com/read/7533/effects-of-crude-and-citrullus-lanatus-seed-oil-on-some-heamatological-indices-pcv-wbc-rbc-and-platelets-of-wistar-albino-rats-2668>.
"Effects Of Crude And Citrullus Lanatus Seed Oil On Some Heamatological Indices (pcv, Wbc, Rbc And Platelets) Of Wistar Albino Rats.." ProjectStoc.com. 04, 2016. Accessed 04, 2016. https://projectstoc.com/read/7533/effects-of-crude-and-citrullus-lanatus-seed-oil-on-some-heamatological-indices-pcv-wbc-rbc-and-platelets-of-wistar-albino-rats-2668.

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