Production Of Ethanol From Cassava Peels Using Zymomonas Mobilis And Saccharomyces Cerevisiae

ABSTRACT

The yield of bioethanol from cassava peels hydrolysed with Aspergillus niger and crude enzymes (amylase, cellulase and pectinase) was examined in this study. Cassava peels were pretreated by soaking and a combination of soaking and boiling which removes 67% of cyanide. After hydrolysis, Zymomonas mobilis and Saccharomyces cerevisiae were used to ferment the hydrolysates separately at room temperature for five (5) days. The result showed that pretreatment by soaking and boiling for up to 120 minutes removed the highest amount of cyanide and increased carbohydrate but reduced the fibre content (37.04±0.01mg/g, 71.42±0.02%, and 10.38±0.42%). Hydrolysis using Aspergillus niger yielded up to 95.44±0.11mg/g reducing sugar while hydrolysis using enzymes yielded up to 72.38±0.06mg/g reducing sugar.  Highest ethanol yield when the hydrolysates were fermented with Zymomonas mobilis and Sacchromyces cerevisiae were 3% and 2% respectively. Proximate composition of the fermented waste showed 6.37±0.08, 2.71±0.03, 25.49±0.01, 8.97±0.02 and 34.41±0.16% ash, fat, protein, crude fibre and carbohydrate respectively while nitrogen, phosphorus and potassium of the residue was 4.08±0.50, 0.40±0.28 and1.84±0.15% respectively. The work reveals that cassava peels is a suitable agricultural waste for bioethanol production and fermentation with Zymomonas mobilis yielded high ethanol compare to Saccharomyces cerevisiae. The work also proved that the waste product of fermentation may be used as animal feedstock or fertilizer to enrich the soil for plant growth.

Table of content

RESEARCH AIM AND OBJECTIVES

FERMENTED CASSAVA PEELS AS ANIMAL FOOD SUPPLEMENTS

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