ABSTRACT
Type 2 diabetes mellitus (T2DM) is a chronic progressive disease that has continued to be a global heath and economic burden. Acarbose is an antidiabetic drug, which acts by inhibiting alpha amylase and alpha glucosidase; while gallic acid is a simple phenolic acid that is
widespread in plant foods and beverages such as tea and wine.This study therefore, sought to investigate the influence of gallic acid on α-amylase and α-glucosidase inhibitory and antioxidant properties of acarbose (in vitro). Aqueous solution of acarbose and gallic acid were prepared to a final concentration of 25µM each. Thereafter, mixtures of the samples (50% acarbose + 50% gallic acid; 75% acarbose + 25% gallic acid; 25% acarbose + 75% gallic acid) were prepared.
The results showed that the combination of 50% acarbose and 50% gallic acid showed the highest α-glucosidase inhibitory effect, while 75% acarbose + 25% gallic acid showed highest α amylase inhibitory effect. Furthermore, all the samples caused the inhibition of Fe2+-induced lipid peroxidation (in vitro) in rat pancreatic tissue homogenate, with the combination of 50% acarbose and 50% gallic acid causing the highest reduction in the malondialdehyde content. In addition, all the samples showed antioxidant properties (ferric reducing property, 2, 2'-azino-bis (-3-ethylbenzthiazoline-6-sulphonate (ABTS*) and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radicals scavenging abilities, and Fe2+ chelating ability). Therefore, the combinations of gallic acid with acarbose could be employed in the management of T2DM with the comparative advantage of possible reduction of the side effects of acarbose; nevertheless the combination of 50% acarbose and 50% gallic acid seems the best combinatory therapy for the management of type 2 diabetes mellitus.
TABLE OF CONTENT
CONTENTS PAGES
Title page
Certification
Dedication
Acknowledgement
Table of Content
List of Figures
List of Tables
Abstract
CHAPTER ONE
Introduction
Justification
Objectives
CHAPTER TWO
Literature review
Diabetes Mellitus 5
Pathophysiology of Diabetes Mellitus 5
Classification of Diabetes Mellitus
Type 1 diabetes
Type two diabetes
Prevalence and incidence of type 2 diabetes
Key enzymes linked to type-2 diabetes mellitus
Alpha glucosidase inhibitors
Alpha amylase inhibitors
Free radicals and oxidative stress
Hyperglycemia and oxidative stress
Lipid peroxidation
Antioxidants
Endogenous antioxidants
Dietary antioxidants
Polyphenols
Gallic acid
Acarbose
CHAPTER THREE
Materials Methods
Materials
Sample collection
Aqueous preparation
Chemicals and reagents
Methods
Alpha glucosidase inhibition assay
Alpha amylase inhibition assay
Fe2+ chelation assay
Inhibition of lipid peroxidation and thiobarbituric acid reactions
Determination of ferric reducing antioxidant property
-azino-bis(-3-ethylbenzthiazoline-6-sulphonate scavenging ability , 1-diphenyl–2-picrylhydrazyl (DPPH*) free radical scavenging ability
Data Analysis
CHAPTER FOUR
Results
CHAPTER FIVE
Discussion
CHAPTER SIX
Conclusion and recommendation
Conclusion
Recommendation
REFERENCES
APPENDIX
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CITE THIS WORK
(2016, 08). Influence Of Gallic Acid On A-amylase And A-glucose Inhibitory And Antioxidant Properties Of Acarbose.. ProjectStoc.com. Retrieved 08, 2016, from https://projectstoc.com/read/7909/influence-of-gallic-acid-on-a-amylase-and-a-glucose-inhibitory-and-antioxidant-properties-of-acarbose-6651
"Influence Of Gallic Acid On A-amylase And A-glucose Inhibitory And Antioxidant Properties Of Acarbose." ProjectStoc.com. 08 2016. 2016. 08 2016 <https://projectstoc.com/read/7909/influence-of-gallic-acid-on-a-amylase-and-a-glucose-inhibitory-and-antioxidant-properties-of-acarbose-6651>.
"Influence Of Gallic Acid On A-amylase And A-glucose Inhibitory And Antioxidant Properties Of Acarbose.." ProjectStoc.com. ProjectStoc.com, 08 2016. Web. 08 2016. <https://projectstoc.com/read/7909/influence-of-gallic-acid-on-a-amylase-and-a-glucose-inhibitory-and-antioxidant-properties-of-acarbose-6651>.
"Influence Of Gallic Acid On A-amylase And A-glucose Inhibitory And Antioxidant Properties Of Acarbose.." ProjectStoc.com. 08, 2016. Accessed 08, 2016. https://projectstoc.com/read/7909/influence-of-gallic-acid-on-a-amylase-and-a-glucose-inhibitory-and-antioxidant-properties-of-acarbose-6651.
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